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Antibody(secreted proteins)|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody(secreted proteins)

【A】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
APPc2011-08-10 Amyloidb1-40 1-42WBIF IHCIP
IBLx1000
18580/582?
rabbit

mouse albumin2011-08-10 WBIF IHCIP
Cappelx100
55442
3%PFA (in PBS)
rabbit
p

マウス培養肝臓細胞
GABA2011-08-10 g-aminobutyric acidWBIF IHCIP
Sigmax100
A2052
3%PFA (in PBS)
rabbit
IFではよくわからないが、ImEMではシナプス小胞が染まっているように見える
By ImEM, synaptic vesicles can be stained by this Ab, but staining is not evident by IF.
マウス脳
α-Amylase2011-08-10 WBIF IHCIP
Sigmax100
A8273
3%PFA (in PBS)
rabbit
p

マウス膵臓
amyloid b protein2011-08-10 WBIF IHCIP
Chemiconx100
MAB1560
3%PFA (in PBS)
mouse
m

neuro2A細胞
Alfa fetoprotein2011-09-22 WBIF IHCIP
Lab Visionx250
RB-365
rabbit
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
poly

mouse E6.5 embryo.
amylase2011-08-10 C-20WBIF IHCIP
Santa Cruz
sc12821
goat
p

【B】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
BiP2012-01-25 WBIF IHCIP
BD Transductionx100
G73320
3%PFA (in PBS)
mouse
0.05%saponin
IgG2a

MDCK細胞

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
anti-Clara Cell Secretory Protein2011-01-30 WBIF IHCIP
millipore
07-623
3%PFA
rabbit

抗原の賦活化が必要らしい
Antigen retreival should be required
マウス肺
ChromograninB2011-08-14 WBIF IHCIP
BD transduction
611646
mouse
m

Cerl2011-09-22 WBIF IHCIP
R&D systems1/100
AF1075
goat
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
poly

mouse E6.5 embryo
Calnexin2012-01-25 WBIF IHCIP
BD Transductionx100
C45520
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
calnexin2012-06-21 WBIF IHCIP
BD Transduction Labx100
C45520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
calnexin2012-06-21 WBIF IHCIP
BD Transduction Labx100
C45520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
Clara cell secretory protein (CCSP)2012-02-22 WBIF IHCIP
Seven Hills Bioreagentsx100
WRAB-3950
3%PFA in 0.1M phosphate buffer (pH7.2)
rabbit
還流固定
なじみのない会社なので注文には時間がかかるかもしれません
Ordering may take time because this Ab is produced by a small company.
mouse lung frozen section

【D】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
dopamine2011-08-10 WBIF IHCIP
Chemicon
AB122S
rabbit
p

dopamine beta hydroxylase2011-08-10 DBHWBIF IHCIP
Chemicon
MAB308
mouse
m

dopamine transporter2011-08-10 DTWBIF IHCIP
millipore
MAB369
rat
m

試してない?
Not used

【E】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
ERp722012-06-21 WBIF IHCIP
BD Transduction Labx100
E72920
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
ERp722012-06-21 WBIF IHCIP
BD Transduction Labx100
E72920
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK

【G】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
glucagon2015-08-07 monoclonal AbWBIF IHCIP
Cell signaling400
8223
3%PFA (in PBS)
rabbit

mouse pancreas
mouse galectin-42011-08-10 WBIF IHCIP
R&D Systems
AF2128
3%PFA (in PBS)
goat
(diffuse stain)
IFOK?
マウス小腸
Glucagon2011-08-10 clone:K79bB10WBIF IHCIP
Sigmax100
G2654
3%PFA (in PBS)
mouse
m

マウス膵臓
GM1302012-01-25 WBIF IHCIP
BD Transductionx100
G65120
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
GS152012-01-25 WBIF IHCIP
BD Transductionx100
G72420
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
GS272012-01-25 WBIF IHCIP
BD Transductionx100
G76220
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
Golgin842012-01-25 WBIF IHCIP
BD Transductionx100
G93720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
rat growth hormone2011-08-10 GHWBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACRT2501RBP85
3%PFA (in PBS)
rabbit

マウス下垂体

【H】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
human HB-EGF2011-10-12 H6WBIF IHCIP
Santa Cruz11抗血清1:50
【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定
Fix the freshly frozen sections with cold acetone
rabbit
(polyclonal)

【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】G54-R73/pro-domain
【IF】HB-EGF強制発現Vero細胞【IHC】種々のヒト生検組織
Mol.Biol.Cell1995;6:967-980;J Histochem Cytochem 2001;49:439-444
human HB-EGF2011-10-12 M6WBIF IHCIP
Santa Cruz
rabbit
(polyclonal)

【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E195-H208/CTF
mekada, unpublished
human HB-EGF2011-10-12 3H4WBIF IHCIP
Bio Academia10.10.1
【IF】4%PFA
mouse
Mono,IgG1
【WB】非還元のみ反応【増殖因子活性阻害】(-)【シェディング阻害】(+++):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
【IF】HB-EGF強制発現Vero細胞
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 1F3WBIF IHCIP
Bio Academia10.12
【IF】4%PFA
mouse
Mono,IgG1
【WB】非還元のみ反応【増殖因子活性阻害】(-)【Dt結合阻害】(+++):3ug/ml【シェディング阻害】(+++):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
【IF】HB-EGF強制発現Vero細胞
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 5D9WBIF IHCIP
Bio Academia1100.1
【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定
Fix the freshly frozen sections with cold acetone
mouse
Mono,IgG2a
【増殖因子活性阻害】(-)【Dt結合阻害】(+++):3ug/ml【シェディング阻害】(++):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
【IF】HB-EGF強制発現Vero細胞【IHC】ヒト子宮頸癌生検組織
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 7E10WBIF IHCIP
Bio Academia0.12
【IF】4%PFA
mouse
Mono,IgG1
【増殖因子活性阻害】(-)【Dt結合阻害】(+++):3ug/ml【シェディング阻害】(++):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】I133/H135/EGF-domain
【IF】HB-EGF強制発現Vero細胞
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 3D9WBIF IHCIP
Bio Academia0.12
【IF】4%PFA
mouse
Mono,IgG1
【増殖因子活性阻害】(-)
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】I133/H135/EGF-domain
【IF】HB-EGF強制発現Vero細胞
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 6C11WBIF IHCIP
Bio Academia1102
【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定
Fix the freshly frozen sections with cold acetone
mouse
Mono,IgG2a
【増殖因子活性阻害】(-)【シェディング阻害】(+):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】N-upstream of EGF-domain
【IF】HB-EGF強制発現Vero細胞【IHC】ヒト子宮頸癌生検組織
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 KM3566WBIF IHCIP
Kyowa Hakko Kirin111
【IF】4%PFA
mouse
Mono,IgG1
【増殖因子活性阻害】(+++):30ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】EGF-domain
【IF】HB-EGF強制発現Vero細胞
Clin.CancerRes.in press
monkey CD92011-10-12 7WBIF IHCIP
11抗血清1:1001
mouse
Mono,IgG2a
【WB】非還元のみ反応【Dt結合阻害】(++):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】2nd loop
【IF】Vero細胞など【IHC】種々のヒト生検組織
J.Biol.Chem.1991;266:20463-20469;J Histochem Cytochem 2001;49:439-444
Histamine(合成ヒスタミン synthetic histamine)2011-11-26 WBIF IHCIP
ImmunoStarx250
22939
カルボジイミド固定
Carbodiimide fixation
rabbit

Drosophila embryo, discs, nervous system
human HB-EGF2011-10-12 4G10WBIF IHCIP
Bio Academia10.10.1
71-501
【IF】4%PFA
mouse
Mono,IgG1
【WB】非還元のみ反応【増殖因子活性阻害】(-)【Dt結合阻害】(+++):3ug/ml【シェディング阻害】(+++):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】E141/EGF-domain
【IF】HB-EGF強制発現Vero細胞
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 Anti-human HB-EGF Neutralizing AntibodyWBIF IHCIP
R&D0.251
AF-259-NA
【IF】4%PFA
goat
(polyclonal)
【増殖因子活性阻害】(+++):1ug/ml【シェディング阻害】(-)
【種特異性 species specificity】human(+++)/mouse(+)【エピトープ epitope】ectodomain
【IF】HB-EGF強制発現Vero細胞
Hsp902012-01-25 WBIF IHCIP
BD Transductionx100
H38220
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
Hsp902012-06-21 WBIF IHCIP
BD Transduction Labx100
H38220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
Hsp902012-06-21 WBIF IHCIP
BD Transduction Labx100
H38220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
Hsp1102012-06-21 WBIF IHCIP
BD Transduction Labx100
H44620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
Hsp1102012-06-21 WBIF IHCIP
BD Transduction Labx100
H44620
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
Hsp702012-01-25 WBIF IHCIP
BD Transductionx100
H53220
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
Hsp702012-06-21 WBIF IHCIP
BD Transduction Labx100
H53220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
Hsp702012-06-21 WBIF IHCIP
BD Transduction Labx100
H53220
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
HSF42012-06-21 WBIF IHCIP
BD Transduction Labx100
H65520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
HSF42012-06-21 WBIF IHCIP
BD Transduction Labx100
H65520
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
human HB-EGF2011-10-12 MAB259WBIF IHCIP
R&D11100.1
MAB259
【IF】4%PFA【IHC】未固定凍結切片・冷アセトン固定
Fix the freshly frozen sections with cold acetone
mouse
Mono,IgG2a
【WB】非還元のみ反応【増殖因子活性阻害】(+):10ug/ml【Dt結合阻害】(+):30ug/ml【シェディング阻害】(+):10ug/ml
【種特異性 species specificity】human(+)/mouse(-)【エピトープ epitope】H135/EGF-domain
【IF】HB-EGF強制発現Vero細胞【IHC】ヒト子宮頸癌生検組織
J.Biochem.2010;148:55-69
human HB-EGF2011-10-12 C18WBIF IHCIP
Santa Cruz0.2
sc-1413
goat
(polyclonal)

【種特異性 species specificity】human(+++)/mouse(+)【エピトープ epitope】CTF
mouse HB-EGF2011-10-12 M18WBIF IHCIP
Santa Cruz0.2
sc-1414
goat
(polyclonal)

【種特異性 species specificity】human(+)/mouse(+++)【エピトープ epitope】CTF

【I】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Insulin2011-08-10 WBIF IHCIP
Sigmax100
I2018
3%PFA (in PBS)
mouse
m

マウス膵臓
Insulin2018-07-12 WBIF IHCIP
Cell Signaling1/10001/1001/400
L6B10
Mouse
IgG2a
Proinsulin is strongly hybridized with this antibody.
MIN6

【L】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
ovine LHb2011-08-10 WBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACOV27b01RBP85
3%PFA (in PBS)
rabbit

マウス下垂体
Lefty-12011-09-22 WBIF IHCIP
R&D systems4 ug/ml
MAB9941
rat
4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
Mab IgG2A

mouse E6.5 embryo
human lysozyme2011-08-10 WBIF IHCIP
DakoCytomationx100
N1515
3%PFA (in PBS)
rabbit
p
Paneth cells are stained
マウス小腸

【M】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
MSH2011-08-10 WBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACAA2201RBP84
3%PFA (in PBS)
rabbit

マウス下垂体

【P】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
rat prolactin2011-08-10 PRLWBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACRT2601RBP85
3%PFA (in PBS)
rabbit

マウス下垂体
PDI2012-01-25 WBIF IHCIP
BD Transductionx100
P71720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
PDI2012-06-21 WBIF IHCIP
BD Transduction Labx100
P71720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
PDI2012-06-21 WBIF IHCIP
BD Transduction Labx100
P71720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
pro-SPC2012-02-21 WBIF IHCIP
Seven Hills Bioreagentsx100
WRAB-9337
3%PFA in 0.1M phosphate buffer (pH7.2)
rabbit
還流固定
なじみのない会社なので注文には時間がかかるかもしれません
Ordering may take time because this Ab is produced by a small company.
mouse lung frozen section

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
slit2011-11-26 C55S.6DWBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse

Drosophila embryo
synaptobrevin/VAMP 22011-08-10 WBIF IHCIP
Synaptic Sy
104211
mouse
m

human somatostatin2011-08-10 WBIF IHCIP
Biogenesisx100
83300009
3%PFA (in PBS)
rat
m

マウス膵臓

【s】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
SP-D2015-01-13 WBIF IHCIP
Milliporex2500
AB3434
Mouse

mouse lung frozen section

【S】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
human synaptobrevin2011-08-10 VAMPWBIF IHCIP
Chemicon
MAB335
mouse
m

bovine synaptophysin2011-08-10 WBIF IHCIP
Chemicon
MAB5258
mouse
m

syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma5000
S0664
mouse

pancreas lysate
syntaxin12015-10-06 HPC-1WBIF IHCIP
Sigma100
S0664
3%PFA (in PBS)
mouse

mouse primary neuron, neural progenitor cell, pancreas
Syntaxin62012-01-25 WBIF IHCIP
BD Transductionx100
S55420
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
Serotonin, 5-HT2011-11-26 S5545WBIF IHCIP
sigmax200
S5545
4%PFA +0.1%Triton
rabbit

Drosophila nervous system
STI1/Hop-p602012-01-25 WBIF IHCIP
BD Transductionx100
S65720
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞
STI-12012-06-21 WBIF IHCIP
BD Transduction Labx100
S65720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
Caco2
STI-12012-06-21 WBIF IHCIP
BD Transduction Labx100
S65720
3%PFA in PBS
mouse
0.05% saponin
IgG1
Ab in Chaperone sampler kit (BD Transduction Lab)
MDCK
SP-C2011-08-10 C-19WBIF IHCIP
Santa Cruz
sc7705
goat
p

試してない?
Not used

【T】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
trachea2011-11-26 2A12WBIF IHCIP
Developmental study hybridoma bank
4%PFA +0.1%Triton
mouse IgM
IgM

Drosophila trachea
rat thyrotropin2011-08-10 TSHWBIF IHCIP
群大生体調節研 IMCR, Gunma Univx100
HACRT2901RBP86
3%PFA (in PBS)
rabbit

マウス下垂体
mouseαTyrosine Hydroxylase2011-08-10 WBIF IHCIP
milliporex100
MAB318
3%PFA (in PBS)
mouse
m

マウス脳

【V】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
VAMP3 (cellubrevin)2015-06-02 WBIF IHCIP
synaptic systemsx1000x100
104102
3%PFA (in PBS)
rabbit
For WB, 20ug of neural progenitor cell lysate was used
mouse primary neuron, neural progenitor cell
Vti1a2012-01-25 WBIF IHCIP
BD Transductionx100
V85620
3%PFA (in PBS)
mouse
0.05%saponin
IgG1

MDCK細胞

【W】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Wg2011-11-26 4D4WBIF IHCIP
Developmental study hybridoma bank S
4%PFA +0.1%Triton
mouse
IgG

Drosophila embryo, discs, nervous system